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Isolation Identification & Molecular Based Investigation Of Bovine Rotavirus

by Ambreen Masood | Prof. Dr. Tahir Yaqoob | Dr. Jawad Nazir | Dr. Sehrish Firyal.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Livestock is an important part of the economy of Pakistan. Calf's diarrhea due to group A bovine rotavirus causes high morbidity and mortality, which results in significant economic losses to livestock. In Pakistan overall prevalence of bovine rotavirus infection is 2.6%. As Pakistan is a developing country, survival of calves is really important to produce milk, meat and hides for propagation of livestock. The aim of current study was to isolate bovine rotavirus from faecal samples of diarrheic calves by antigen capture ELISA and molecular investigations. So, it was helpful to check the prevalence of bovine rotavirus in Lahore district. This study will be a milestone for better treatment strategies of calf diarrhea problem. It will also pave the way for better vaccine development strategies to cure the disease. A total of 100 diarrheic faecal samples of cattle and buffalo's calves less than three months of age were collected from Lahore district. Rotavirus screening was done by direct sandwich ELISA by using commercial Rotavirus detection kit (Cypress Diagnostics, Belgium). ELISA confirmed 12 samples to be positive for bovine rotavirus. Among 12 positive samples, 7 were found positive in buffalo calf and 5 in cattle calf. After RNA extraction and cDNA synthesis, the PCR was done for amplification of VP4 gene of all ELISA positive bovine rotavirus samples. But only 5 samples (3 buffalo calf samples and 2 cattle calf samples) give desired product of 880 bp of VP4 gene. After sequencing and bioinformatics analysis, phylogenetic tree was constructed. It is evident that Pakistani bovine rotavirus VP4 gene (BRV/QOL/13) has maximum identity of 98% with Indian bovine rotaviruses VP4 gene. Availability: Items available for loan: UVAS Library [Call number: 1707,T] (1).

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Genetic Diversity Among Different Isolates Of Pasteurella Multocida From Poultry

by Arslan Sardar (2013-VA-282) | Dr. Imran Altaf | Prof. Dr. Aftab Ahmad Anjum | Dr. Sehrish Firyal.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Fowl cholera is an acute bacterial disease of broiler breeders and layer breeders caused by Pasteurella multocida. In the present study, 10 isolates from different areas of Punjab were purified. These samples were confirmed by API Kit. Different molecular techniques like PCR and RFLP were used to investigate variation at the molecular level among 10 isolates collected from different areas of Punjab. Different mutations were observed among 10 field isolates at different mutation sites by sequencing. Phylogentic tree was also made using MEGA6 software that supported the sequencing results. ‘Msp1’ endonuclease cleaved bacterial whole genome at different cutting sites, all 10 isolates collected from different districts of Punjab cleaved into 3 to 5 fragments ranging from 600 to 10000 base pairs which showed the genetic variation among 10 isolates of P.mulocida. Availability: Items available for loan: UVAS Library [Call number: 2315-T] (1).

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